Monday, 30/11/2020

PROTRANS HLA NGS

Next Generation Sequencing (NGS)

PROTRANS N5



Highlights of Protrans N5

 

1

Single PCR Next Generation Sequencing:

Amplification and sample indexing in one PCR

 

2

Only one purification needed

 

3

No need of fragmentation

 

4

Short Sample to sequencing time

Low hands-on time

Easy to handle

 

5

High and consistent coverage

Optimal for low and high throughput

 

 

Easiest Workflow

  • One short and robust PCR for the complete workflow
  • Amplification and Index Labeling in the same PCR
  • Same protocol for all loci
  • One Purification of pooled PCR products for the entire workflow
  • No need of Fragmentation

 

Fastest Turnaround Time

  • Minimized hands-on time of less than 1.5 h
  • Sample to sequencing in less than 4.5 h
  • Sample to high-resolution result in 1.5 days

 

Efficient NGS Strategy

  • Sequencing the key regions of the HLA genes
  • Null Alleles are covered
  • Allele drop-outs are minimized
  • Optimum use of the flow cell capacity
  • High and consistent coverage of the Amplificates

 

Scalable Solutions

  • Optimal for low and high throughput
  • Less than 0.5 μg of starting gDNA from Blood, Saliva or Buccal Swab
  • Minimized hands-on time makes automation unnecessary
  • For highest throughput, automation is easy to implement

 

Reliable Data Analysis

  • User-friendly and reliable Software
  • Fast analysis and easy-to-interpret results
  • No need for high performance computing power

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