PROTRANS HLA NGS
Next Generation Sequencing (NGS)
PROTRANS N5
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Highlights of Protrans N5
1
Single PCR Next Generation Sequencing:
Amplification and sample indexing in one PCR
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Only one purification needed
3
No need of fragmentation
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Short Sample to sequencing time
Low hands-on time
Easy to handle
5
High and consistent coverage
Optimal for low and high throughput
Easiest Workflow
- One short and robust PCR for the complete workflow
- Amplification and Index Labeling in the same PCR
- Same protocol for all loci
- One Purification of pooled PCR products for the entire workflow
- No need of Fragmentation
Fastest Turnaround Time
- Minimized hands-on time of less than 1.5 h
- Sample to sequencing in less than 4.5 h
- Sample to high-resolution result in 1.5 days
Efficient NGS Strategy
- Sequencing the key regions of the HLA genes
- Null Alleles are covered
- Allele drop-outs are minimized
- Optimum use of the flow cell capacity
- High and consistent coverage of the Amplificates
Scalable Solutions
- Optimal for low and high throughput
- Less than 0.5 μg of starting gDNA from Blood, Saliva or Buccal Swab
- Minimized hands-on time makes automation unnecessary
- For highest throughput, automation is easy to implement
Reliable Data Analysis
- User-friendly and reliable Software
- Fast analysis and easy-to-interpret results
- No need for high performance computing power
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