PROTRANS HLA NGS

Next Generation Sequencing (NGS)

by PROTRANS N5 and N5LR11

Highlights of Protrans N5 and N5LR11

 

1

Single PCR Next Generation Sequencing

 

2

Only one purification needed

 

3

Strong reduction of allele drop-outs

 

4

Short Sample to sequencing time

Low hands-on time

Easy to handle

 

5

High and consistent coverage

Optimal for low and high throughput

 

 

Easiest Workflow

• One short and robust PCR for the complete workflow
• Amplification and Index Labeling in the same PCR (N5)
• Same protocol for all loci
• One Purification of pooled PCR products for the entire workflow
• No need of Fragmentation (N5)

 

Fastest Turnaround Time

• Minimized hands-on time ~ 1 h
• Sample to Sequencer in one working day (~8h)
• Sample to high-resolution result in 1.5 days

 

Efficient NGS Strategy

• Sequencing the key regions (N5) or
  
• Complete HLA genes (N5 LR11)
• Null Alleles are covered
• Allele drop-outs are minimized
• Optimum use of the flow cell capacity
• High and consistent coverage of the Amplificates

 

Scalable Solutions

• Optimal for low and high throughput
• Less than 0.5 μg of starting gDNA from Blood, Saliva or Buccal Swab
• Minimized hands-on time makes automation unnecessary
• For highest throughput, automation is easy to implement
• Wide compatibility for N5 LR11 (Illumina MiSeq/MiniSeq/iSeq, ONT MinION)
  

 

Reliable Data Analysis

• User-friendly and reliable Software
• Fast analysis and easy-to-interpret results
• No need for high performance computing power

Products:

• Protrans N5
• Protrans N5 LR11